Fig. 2

Effects of IO on the morphologies and areas of pancreatic islets in high-fat diet (HFD)-fed mice model. a Proliferation marker of cell (proliferating cell nuclear antigen, PCNA) was evaluated by immunohistochemistry, and b quantitative graph shows the PCNA expression level on pancreatic islets from representative images. c Hematoxylin and eosin (H&E)-stained pancreatic islets (round shape and pale color) and d the size of pancreatic islets were measured by the Image J software from representative images (scale bar = 100 μm). Data are presented as mean ± S.D. **p < 0.01 vs NFD/saline group; ^#p < 0.05, ^##p < 0.01, or ^###p < 0.001 vs HFD/saline group