Table 2 Microsatellite loci used for mangrove red snapper
Locus | Repeat motif | Primer sequences | Tm (°C) | Size (bp) | Accession no. |
|---|---|---|---|---|---|
LA02_M13 | (AG)n | F: GATAACAATTTCACACAGG-GTATCACGATGTCTCAGCCAGT R: GTTTCTT-CAGTTCTAAGCGGTTTCTCAAG | 56-60 | 161-193 | DQ219295 |
LA04_T3 | (AC)n | F: AATTAACCCTCACTAAAGGG-GAGGCTGTAGTGCTCTGCCC R: GTTTCTT-GTTCACCTTCATGGCGACAG | 56-60 | 255-317 | DQ219297 |
LA05_Hill | (TGC)n | F: TGACCGGCAGCAAAATTG-GAGATTTTGGAATGCTGTG R: GTTTCTT-AGTAACCACTGTCTCTGCA | 56-60 | 157-159 | DQ247923 |
LA06_Neo | (TGC)n | F: AGGTGAGATGACAGGAGATC-CCTGATCATAGTGACGCGC R: GTTTCTT-AATCGGAATCGGTTCATCTC | 56-60 | 159-169 | DQ247924 |