Table 1 Optimal conditions for enzymatic hydrolysis of various enzymes
Enzyme | Buffer | pH | Temperature (°C) |
|---|---|---|---|
Alcalase | 50 mM Na2HPO4-NaH2PO4 | 7.0 | 50 |
α-Chymotrypsin | 50 mM Na2HPO4-NaH2PO4 | 7.0 | 37 |
Neutrase | 50 mM Na2HPO4-NaH2PO4 | 7.0 | 50 |
Papain | 50 mM Na2HPO4-NaH2PO4 | 7.0 | 37 |
Pepsin | 20 mM HCl | 2.0 | 37 |
Trypsin | 50 mM Na2HPO4-NaH2PO4 | 7.0 | 37 |