Table 2 Primer sequences for MLST analysis
Primer | Sequence (5′–3′) | Annealing temperature | Expected size |
|---|---|---|---|
rpoD-F | GCCGAAGAAGAATTTGACCTTAA | 54 °C | 854 bp |
rpoD-R | CGTTTRCTTCTGCTHGGATGTCT | ||
glpF-F | WTGACAGCATTTTGGGG | 54 °C | 690 bp |
glpF-R | GTAAAATACRCCGCCGA | ||
ilvD-F | ATGAGATATTCGCTGCC | 54 °C | 622 bp |
ilvD-R | CTTCGTTAATGCGTTCTAAAGAG | ||
ptA-F | ATACATATGAAGGSATGGAAGA | 54 °C | 610 bp |
ptA-R | TAGCCGATGTTTCCTGCT | ||
tpiA-F | TCAGCTTCGTTGAAGAAGTGAAA | 54 °C | 620 bp |
tpiA-R | GGACTCTGCCATATATTCTTTA | ||
PycA-F | AAATCAGARGCGAAAGC | 54 °C | 545 bp |
PycA-R | CCTGAGCGGTAAGCCAT | ||
purH-F | TTTGAGAAAAAACAATCGCT | 54 °C | 568 bp |
purH-R | TCGGCTCCCTTTTCGTCGG |