Fig. 5

Inhibitory effect of D. puetteri ethanolic extract (DPE) on melogenic gene expression in α-MSH-stimulated B16F10 melanoma cells. Effect of DPE on a tyrosinase and b MITF gene expression. Cells were exposed to 100 nM α-MSH in the presence and absence of DPE (200 μg/mL) and PD98059 (10 μM). Tyrosinase and MITF mRNA levels were examined by real-time RT-PCR using GAPDH as an internal control. Results represent the pooled mean ± SE of three independent experiments, performed in triplicate. *p < 0.05, **p < 0.01