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Fig. 5 | Fisheries and Aquatic Sciences

Fig. 5

From: The pepsinolytic hydrolysate from Johnius belengerii frame inhibited LPS-stimulated production of pro-inflammatory mediators via the inactivating of JNK and NF-κB pathways in RAW 264.7 macrophages

Fig. 5

Inhibitory effect of JFH on LPS-induced phosphorylation of I-κB and NF-κB in RAW264.7 macrophages. Cells were stimulated with LPS (0.25 μg/ml) in the presence of JFH (10, 50, and 100 μg/ml) for 30 min. Nuclear fraction (NF-κB) and cytosol fraction (I-κB and NF-κB) were measured by Western blotting. The density ratio of the protein expressed in the untreated group was considered to represent 1-fold. The data is expressed as mean folds of the protein expression versus LPS-treated group. Values are expressed as means ± S.D. of triplicate experiments. *p < 0.05 indicates significant differences from the untreated group

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