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Table 2 PCR and real-time PCR results for detecting K. septempunctata in orbiniid polychaete N. laevigata isolated from outflow waterway of fish tank

From: Molecular detection of Kudoa septempunctata (Myxozoa: Multivalvulida) in sea water and marine invertebrates

  PCR Positive samples/tested samples (%) Real-time PCR
Ct value rDNA copy number/ body weight (g)
May 36/90 (40.0%) 38.9–41.4 2.5 × 103–1.6 × 103
June 14/62 (22.6%) 35.5–38-3 2.0 × 103–2.1 × 104
July 5/126 (4.0%) nd nd
August 0/84 nd nd
September 0/82 nd nd
October 0/62 nd nd
November 0/72 nd nd
  55/578 (9.5%)   
  1. nd not detected