Fig. 4

Expression of abalone MTF-1 in developing embryos and early larvae assessed by RT-qPCR analysis using RPL7 and PPL8 reference genes as normalization controls. Abbreviations for developmental samples are unfertilized eggs (UF), eggs at early cleavages (EC; 2~8 cells stage), eggs at morula (MO), trochophore (TR), early veliger (EV), and late veliger (LV). Histograms with different letters indicate the significantly different means based on ANOVA followed by Duncan’s multiple ranged tests at P < 0.05