Table 1 Oligonucleotide primers used in PCR amplification of ARF1b of P. olivaceus; F, Forward; R, reverse
Primer name | 5'-3' sequence | Information |
|---|---|---|
DgARF1b-F1 | ATGGGDRMYWTBKCYWSC | Primers for cDNA library screening |
DgARF1b-F2 | GACVACCATCYTGTACAARCTCAAAC | |
DgARF1b-R1 | CAYTTBVYDTTYYTBAGCTKG | |
DgARF1b-R2 | CRTCYTCWGMCARCATTCGCATC | |
3′GSP-PoARF1b-F1 | GTTGAGACAGTAGAGTACAGGAACATCAG | |
3′GSP-PoARF1b-F2 | GGAGCGAATCGGTGAGGCGAGAGAGGAGC | |
5′GSP-PoARF1b R1 | CAGTTCCGCCGGGTGAGGTCGTGCAGGC | |
5′GSP-PoARF1b R2 | GCTCCTCTCTCGCCTCACCGATTCGCTCC | |
PoARF1b-RT-F | GCAGCAAAGTACTTCAAAGCCC | Primers for qPCR |
PoARF1b-RT-R | CTCAGCCAGCATTCGCATC | |
Po18S rRNA-RT-For2 | ATGGCCGTTCTTAGTTGGTG | GenBank accession no. EF126037.1 |
Po18S rRNA-RT-Rev2 | CACACGCTGATCCAGTCAGT | |
PoARF1b-ORF F | ATGGGACAGTTCTTTAGCCTGTTTAAAG | Primers for the construction of pEGFP CI |
PoARF1b-ORF R | TCATTTTATGTTCTTGAGCTGGTTTGAAAG | |
Xhol-PoARF1b-F | CCGCTCGAGCTATGGGACAGTTCTTTA | |
Xpnl-PoARF1b-R | CGGGGTACCTCATTTTATGTTCTTGAGCTG | |
PoARF1bT30N-F | CTGCATGCTGGAAAGAACACCATCCTGTACAAA | Primers for the site-directed mutation |
PoARF1bT30N-R | TTTGTACAGGATGGTGTTCTTTCCAGCATGCAG | |
PoARF1bQ70L-F | GTCGGTGGTCTGGACAAGATCAGGCCACTC | |
PoARF1bQ70L-R | GAGTGGCCTGATCTTGTCCAGACCACCGAC |