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Fig. 3 | Fisheries and Aquatic Sciences

Fig. 3

From: Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)

Fig. 3

SDS-PAGE of expressed (a) and purified (b) Pol AFP IV and its TH activity and ice morphology (c). a M protein size marker, lane 1 lysates of uninduced bacteria, lane 2 lysates of bacteria induced with IPTG and lanes 3 and 4 soluble fraction and insoluble fractions of total protein from the induced bacteria, respectively. b M protein size marker, lane 1 elution fraction 1, and lane 2 elution fraction 2. Arrows indicate Pol AFP IV. c Antifreeze activity of recombinant Pol AFP IV. The ice morphology of the Pol AFP IV solution was examined using a nanoliter osmometer under a light microscope

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