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Table 3 Effects of various growth factors on the culture of Sebastes schlegeli ovary-dissociated cells

From: Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Composition of culture medium No. of cell populations tested No. (%)a of cell populations initially attached No. (%)a of cell populations subcultured to
Passage 1 Passage 2
B 6 6 (100) 6 (100) 2 (33)
B + E 3 3 (100) 3 (100) 1 (33)
B + F 3 3 (100) 3 (100) 1 (33)
B + L 3 3 (100) 3 (100) 1 (33)
B + E + F 3 3 (100) 3 (100) 1 (33)
B + E + L 3 3 (100) 3 (100) 1 (33)
B + F + L 3 3 (100) 3 (100) 1 (33)
B + E + F + L 3 3 (100) 3 (100) 1 (33)
B + FS 3 3 (100) 3 (100) 2 (67)
B + MEE 3 3 (100) 3 (100) 2 (67)
B + FS + MEE 3 3 (100) 3 (100) 2 (67)
  1. B, L15 medium containing 20 % fetal bovine serum and 1 % mixed solution of penicillin and streptomycin; E, 25 ng/ml epidermal growth factor; F, 10 ng/ml basic fibroblast growth factor; L, 1000 units/ml leukemia inhibitory factor; FS, 1 % fish serum; MEE, 50 μg/ml medaka embryo extract
  2. aPercentage of the number of cell populations tested