Table 3 Effects of various growth factors on the culture of Sebastes schlegeli ovary-dissociated cells
Composition of culture medium | No. of cell populations tested | No. (%)a of cell populations initially attached | No. (%)a of cell populations subcultured to | |
|---|---|---|---|---|
Passage 1 | Passage 2 | |||
B | 6 | 6 (100) | 6 (100) | 2 (33) |
B + E | 3 | 3 (100) | 3 (100) | 1 (33) |
B + F | 3 | 3 (100) | 3 (100) | 1 (33) |
B + L | 3 | 3 (100) | 3 (100) | 1 (33) |
B + E + F | 3 | 3 (100) | 3 (100) | 1 (33) |
B + E + L | 3 | 3 (100) | 3 (100) | 1 (33) |
B + F + L | 3 | 3 (100) | 3 (100) | 1 (33) |
B + E + F + L | 3 | 3 (100) | 3 (100) | 1 (33) |
B + FS | 3 | 3 (100) | 3 (100) | 2 (67) |
B + MEE | 3 | 3 (100) | 3 (100) | 2 (67) |
B + FS + MEE | 3 | 3 (100) | 3 (100) | 2 (67) |