Table 2 Effects of temperature and basal media on the culture of Sebastes schlegeli ovary-dissociated cells
Factors | Group | No. of cell populations tested | No. (%)a of cell populations initially attached | No. (%)a of cell populations subcultured to | |||
|---|---|---|---|---|---|---|---|
Passage 1 | Passage 2 | Passage 3 | Passage 4 | ||||
Whole replicates | Merged | 42 | 42 (100) | 27 (64) | 9 (21) | 3 (7) | 1 (2) |
Basal media | L15 | 21 | 21 (100) | 18 (86) | 8 (38) | 3 (14) | 1 (5) |
DMEM | 21 | 21 (100) | 9 (43) | 1 (5) | 0 (0) | 0 (0) | |
P-value | - | 0.003 | 0.0076 | 0.0754 | 0.3233 | ||
Temperature | 15 | 14 | 14 (100) | 10 (71) | 4 (29) | 1 (7) | 0 (0) |
(°C) | 20 | 14 | 14 (100) | 9 (64) | 3 (21) | 1 (7) | 0 (0) |
25 | 14 | 14 (100) | 8 (57) | 2 (14) | 1 (7) | 1 (7) | |
P-value | - | 0.7475 | 0.6717 | 1.0000 | 0.3771 | ||