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Table 2 Effects of temperature and basal media on the culture of Sebastes schlegeli ovary-dissociated cells

From: Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Factors Group No. of cell populations tested No. (%)a of cell populations initially attached No. (%)a of cell populations subcultured to
Passage 1 Passage 2 Passage 3 Passage 4
Whole replicates Merged 42 42 (100) 27 (64) 9 (21) 3 (7) 1 (2)
Basal media L15 21 21 (100) 18 (86) 8 (38) 3 (14) 1 (5)
  DMEM 21 21 (100) 9 (43) 1 (5) 0 (0) 0 (0)
  P-value   - 0.003 0.0076 0.0754 0.3233
Temperature 15 14 14 (100) 10 (71) 4 (29) 1 (7) 0 (0)
(°C) 20 14 14 (100) 9 (64) 3 (21) 1 (7) 0 (0)
  25 14 14 (100) 8 (57) 2 (14) 1 (7) 1 (7)
  P-value   - 0.7475 0.6717 1.0000 0.3771
  1. This table was derived from reallocation of data from Table 1
  2. aPercentage of the number of cell populations tested