Effect of water temperature on protein requirement of Heteropneustes fossilis (Bloch) fry as determined by nutrient deposition, hemato-biochemical parameters and stress resistance response

Table 4 Metabolic enzymes activities and hematological parameters of fry Heteropneustes fossilis

Temp. °C	Diet (% protein)	AST (IU/L)	ALT (IU/L)	Hct%	Hb g/dL		RBCs (×10⁹)
18	D₂₈	54.77 ± 0.03^Bb	57.11 ± 0.01^Aa	21.24 ± 0.02^Ee	6.14 ± 0.02^e		2.67 ± 0.02^Dd
	D₃₂	53.11 ± 0.05^Cc	55.23 ± 0.02^Bb	23.97 ± 0.09^Dd	6.98 ± 0.02^d		2.79 ± 0.02^Cc
	D₃₆	52.21 ± 0.02^Dd	54.33 ± 0.05^Cc	27.43 ± 0.07^Cc	7.78 ± 0.02^c		2.81±0.02^Bb
	D₄₀	51.33 ± 0.04^De	54.11 ± 0.03^Cc	32.91 ± 0.09^Aa	8.83 ± 0.02^b		3.11 ± 0.02^Aa
	D₄₄	51.59 ± 0.01^De	54.21 ± 0.02^Cc	32.97 ± 0.01^Aa	9.97 ± 0.02^a		2.98 ± 0.02^Aa
	D₄₈	54.99 ± 0.03^Bb	54.65 ± 0.04^Cc	31.89 ± 0.01^Aa	9.94 ± 0.02^a		2.85 ± 0.02^Bb
	D₅₂	55.68 ± 0.02^Aa	55.98 ± 0.01^Bb	29.78 ± 0.01^Bb	9.84 ± 0.02^a		2.64 ± 0.02^Dd
26	D₂₈	53.99 ± 0.02^Aa	44.93 ± 0.02^Aa	22.23 ± 0.02^Ee	6.91 ± 0.02^e		2.97 ± 0.02^Cc
	D₃₂	52.65 ± 0.02^Ab	44.18 ± 0.03^Aa	25.51 ± 0.09^Dd	7.19 ± 0.02^d		2.99 ± 0.02^Cc
	D₃₆	51.96 ± 0.02^Bc	42.23 ± 0.05^Bb	28.64 ± 0.07^Cc	8.32 ± 0.02^c		3.14 ± 0.02^Bb
	D₄₀	51.14 ± 0.02^Bc	39.18 ± 0.02^Cc	32.93 ± 0.09^Bb	9.24 ± 0.02^b		3.23 ± 0.02^Bb
	D₄₄	49.13 ± 0.02^Ce	33.98 ± 0.04^Ef	33.92 ± 0.01^Aa	10.59 ± 0.02^a		3.97 ± 0.02^Aa
	D₄₈	49.88 ± 0.02^De	35.98 ± 0.03^De	32.97 ± 0.01^Bb	10.21 ± 0.02^a		3.75 ± 0.02^Aa
	D₅₂	50.97 ± 0.02^Dd	36.98 ± 0.01^Dd	31.91 ± 0.01^Bb	10.11 ± 0.02^a		3.88 ± 0.02^Aa
	P value
	Dietary protein	0.003	0.0005	0.001	0.006	0.002	0.0001
	Temperature	0.008	0.0001	0.004	0.003	NS	0.0009
	Dietary protein × temp	NS	NS	NS	NS	NS	NS

AST aspartate amino transferases, ALT alanine amino transferases, Hct hematocrit, Hb hemoglobin, RBCs red blood cells count
Different upper case superscripts indicate significant differences (P < 0.05) between temperatures, whereas different with superscripts indicate significant differences between dietary protein levels; NS non-significant (P > 0.05)

ISSN: 2234-1757