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Table 1 Composition of experimental diets

From: Effect of water temperature on protein requirement of Heteropneustes fossilis (Bloch) fry as determined by nutrient deposition, hemato-biochemical parameters and stress resistance response

Diets

Ingredients (g/100 g)

(D28)

(D32)

(D36)

(D40)

(D44)

(D48)

(D52)

Caseina

26.99

30.85

34.70

38.55

42.41

46.26

50.13

Gelatinb

6.75

7.71

8.67

9.64

10.60

11.57

12.53

Dextrin

40.82

33.98

27.21

20.43

13.65

6.87

0.07

Corn oilc

5

5

5

5

5

5

5

Cod oild

2

2

2

2

2

2

2

Vitamin premixe,f

3

3

3

3

3

3

3

Mineralg

4

4

4

4

4

4

4

α-Cellulose

1.44

3.46

5.42

7.38

9.34

11.3

13.27

Carboxymethyl cellulose

10

10

10

10

10

10

10

Total

100

100

100

100

100

100

100

Proximate analyses (%)

Analyzed crude protein

28.11

31.97

36.21

40.13

43.99

48.15

52.14

Analyzed crude fat

6.95

7.12

7.31

7.14

6.89

7.11

7.13

Gross energyh, kJ/g

17.9

17.9

17.9

17.9

17.9

17.9

17.9

Estimated gross energyi, kJ/g

17.92

17.91

17.93

17.91

17.94

17.92

17.92

Digestible energy, kJ/g

14.53

14.68

14.83

14.98

15.14

15.29

15.45

P/DE ratio mg/kJ

19.27

21.79

24.27

26.70

29.06

31.39

33.66

  1. aCrude protein (80 g/100 g)
  2. bCrude protein (95 g/100 g)
  3. cCod liver oil from SevenSeas Ltd., Hull, UK.
  4. dCorn oil was obtained from Fortune, Adani Wilmar Ltd
  5. eHalver (2002)
  6. fVitamin mixture (1 g vitamin mix +2g Å“-cellulose) choline chloride 0.50; inositol 0.20; ascorbyl-2-polyphosphate 0.10; nicotinic acid 0.075; calcium pantothenate 0.05; riboflavin 0.02; menadione 0.004; pyridoxine hydrochloride 0.005; thiamin hydrochloride 0.005; folic acid 0.0015; biotin 0.0005; alpha-tocopheryl acetate 0.04; vitamin B12 0.00001; Loba Chemie, India
  7. gMineral mixture (g/100 g) calcium biphosphate 13.57; calcium lactate 32.69; ferric citrate 2.97; magnesium sulphate 13.20; potassium phosphate (dibasic) 23.98; sodium biphosphate 8.72; sodium chloride 4.35; aluminium chloride.6H2O 0.0154; potassium iodide 0.015; cuprous chloride 0.010; magnus sulphate. H2O 0.80; cobalt chloride. 6H2O 0.1; zinc sulphate. 7H2O 0.40; Loba Chemie, India
  8. hCalculated on the basis of fuel values 23, 20.19, 16.0, and 37.6 kJ/g for casein, gelatin, dextrin, and fat, respectively, as estimated on Gallenkamp ballistic bomb calorimeter
  9. iEstimated on Gallenkamp ballistic bomb calorimeter