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Table 1 Primer sets used in real time-quantitative PCR

From: Response of body color change rearing under different light intensity conditions in farmed red spotted grouper, Epinephelus akaara

Primer information

Primer

Sequence (5’-3’)

MCH Forward

CTGTTGTCGTCAGCTCTCCG

MCH Reverse

GGGATGCAGATCTACCCACTG

POMC Forward

GAACATCCGAGCTGTCAGGA

POMC Reverse

CATGGAGTAGGAGCGCTTGG

MC1R Forward

GTTGGCCATCTTCAAGAACCG

MC1R Reverse

TCACGTCGATGACGTTGTCC

ASIP Forward

GGCCCACCTCCTGTTGTTATT

ASIP Reverse

GACAGTTCTGAAGAGCCGACA

β-actin Forward

GAGGGGTATCCTGACCCTGA

β-actin Reverse

CTCCTCAGGGGCAACTCTC

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Fisheries and Aquatic Sciences

ISSN: 2234-1757

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