On-farm evaluation of dietary animal and plant proteins to replace fishmeal in sub-adult olive flounder Paralichthys olivaceus

Table 1 Composition of the basal experimental diet for sub-adult olive flounder (% dry matter (DM) basis)

Ingredients	CONT	AP₁₀	APP₂₀
Sardine fish meal	32.47	29.27	26.015
Anchovy fish meal	32.47	29.27	26.015
Soybean meal	14.90	13.80	13.00
Wheat flour	12.00	12.00	12.00
Wheat gluten	1.00	1.00	3.80
SPC¹	0.00	0.00	3.50
Tankage meal	0.00	6.50	3.51
Poultry by-product	0.00	0.00	3.50
Fish oil	3.40	3.20	3.50
Lecithin	0.50	0.50	0.50
Betain	0.00	1.00	1.00
Taurine	0.00	0.20	0.40
MCP	0.50	0.50	0.50
Mineral mix²	1.00	1.00	1.00
Vitamin mix³	0.30	0.30	0.30
ETC⁴	1.46	1.46	1.46
Total	100	100	100
Proximate analysis
Moisture	5.27 ± 0.25	5.27 ± 0.15	5.48 ± 0.08
Crude protein	62.3 ± 0.64	61.3 ± 0.75	62.6 ± 0.57
Crude lipid	8.92 ± 0.01	9.05 ± 0.23	9.56 ± 0.19
Crude ash	11.2 ± 0.01	11.7 ± 0.05	11.4 ± 0.01

¹ SPC soy protein concentrate
²Contains (as mg/kg in diets): Ascorbic acid, 300; dl-Calcium pantothenate, 150; Choline bitate, 3000; Inositol, 150; Menadion, 6; Niacin, 150; Pyridoxine HCl, 15; Rivoflavin, 30; Thiamine mononitrate, 15; dl-a-Tocopherol acetate, 201; Retinyl acetate, 6; Biotin, 1.5; Folic acid, 5.4; Cobalamin, 0.06.
³Contains (as mg/kg in diets): NaCl, 437.4; MgSO₄˙7H₂O, 1379.8; ZnSO₄˙7H₂O, 226.4; Fe-Citrate, 299; MnSO₄, 0.016; FeSO₄, 0.0378; CuSO₄, 0.00033; Calciumiodate, 0.0006; MgO, 0.00135; NaSeO₃, 0.00025.
⁴Ectra (% of mixture): Vitamin C (0.3%), Vitamin E (0.3%), Choline (0.8%), antifungal agent (0.06%)

ISSN: 2234-1757