Fig. 4

Effects of IO on GLUT4 expression increase of skeletal muscle tissues in high-fat diet (HFD)-fed mice model. a Confocal images show GLUT4 (green) expression and nuclei (blue; DAPI) were evaluated of skeletal muscle tissue of all mice groups. b Quantitative graph shows the level of GLUT4 expression from representative images using Zen 2012 software (scale bar = 100 um). c The mRNA levels of Akt, Ampk, and Hk2 were evaluated by qRT-PCR. Data are presented as mean ± S.D. **p < 0.01 vs NFD/saline group; ^#p < 0.05 or ^##p < 0.01 vs HFD/saline group. NS, not significant