Fig. 3
Expression of proteins and genes related to melanogenesis in B16F10 cells against PBE treatment. a Cells were exposed to α-MSH in the presence of 50 and100 μg/mL PBE or 100 μM arbutin, for 72 h. MITF, tyrosinase, TRP-1, and TRP-2 proteins were studied by Western blot. Internal control was β-actin. b Relevant quantitative data. Melanogenic gene expressions. c Tyrosinase and d MITF gene expression. Cells were exposed to α-MSH in the presence and absence of PBE and ERK inhibitor, PD98059. The mRNA levels were examined by RT-PCR. GAPDH was used as an internal control. Results represent as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 (number sign denotes significance compared to control while asterisk represents significance compared to the α-MSH-treated group)