Table 1 Nucleotide sequences of primers used in reverse-transcription polymerase chain reaction (RT-PCR) analysis of adductor muscles of the Pacific oyster Crassostrea gigas
Gene | Accession no. | Sequence (5′–3′) | Amplicon (bp) |
|---|---|---|---|
CIR | AJ535669 | (F)-TGAGGAGGGTGATGAGGATA (R)-ATTGCACTGTAGGGATTGGA | 375 |
IGFBP-ALS | XP_011442465.1 | (F)-CTTGCCCTGAAAACCCTACA (R)-GGGCAAGTAGCAGAATTTGG | 500 |
MIP | XM_011417420.2 | (F)-TAAATACAAGCGGTCGGGTG (R)-AGATCCTTTCTTCTTGCGGC | 214 |
Gyc76C | XM_011452292.2 | (F)-ATGAATGCAGTGAATCGCTTGA (R)-TGATACTGTGCAGCTCTTGG | 274 |
Vg | AB084783.1 | (F)-ATGCTGGAGATGAACCTTGAG (R)-TGCTTCGTGGTGGATATCCT | 273 |
EF1α | AB122066.1 | (F)–CCACTGGCCATCTCATTTAC (R)–TGTTGACACCAATGATGAGC | 393 |