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Table 2 Digestive related genes (NPY, CCK, LepA, and LepB) expression in the brain, liver, and intestine according to the feeding regime

From: Response of appetite-related genes in relation to the rearing water temperature in red spotted grouper (Epinephelus akaara)

Tissue

Gene

Water temperature

Gene expression

+ 0 h

+ 3 h

+ 6 h

− 3 h

Brain

NPY

NC

1.8 ± 0.4

2.9 ± 0.4

1.7 ± 0.3

4.1 ± 0.3*

20 °C

3.8 ± 1.8

1.2 ± 0.2

2.0 ± 0.5

3.2 ± 1.3

25 °C

7.2 ± 1.6

13.3 ± 3.9

4.7 ± 1.1

28.6 ± 8.7*

CCK

NC

0.6 ± 0.1

0.4 ± 0.0

0.4 ± 0.1

0.4 ± 0.1

20 °C

0.3 ± 0.1

0.5 ± 0.0*

0.4 ± 0.1

0.3 ± 0.0

25 °C

0.9 ± 0.2

0.7 ± 0.2

4.1 ± 0.7*

10.3 ± 1.5**

LepA

NC

5.3 ± 1.6

5.8 ± 1.3

2.4 ± 0.5

3.4 ± 0.5

20 °C

1.5 ± 0.9

2.3 ± 0.6*

1.6 ± 0.4

1.9 ± 0.6

25 °C

0.8 ± 0.1

3.3 ± 0.9

2.0 ± 1.0

5.7 ± 2.1

LepB

NC

2.0 ± 0.7

1.5 ± 0.3

0.8 ± 0.1

0.8 ± 0.3

20 °C

1.6 ± 0.4

1.3 ± 0.3

0.8 ± 0.2

0.9 ± 0.3

25 °C

1.5 ± 0.5

1.4 ± 0.1

1.0 ± 0.2

1.3 ± 0.3

Liver

LepA

NC

0.9 ± 0.3

0.5 ± 0.1

0.5 ± 0.1

1.0 ± 0.3

20 °C

0.3 ± 0.1

0.3 ± 0.1

0.8 ± 0.2*

0.3 ± 0.0

25 °C

0.3 ± 0.1

0.4 ± 0.1

0.3 ± 0.0

0.8 ± 0.4

LepB

NC

0.6 ± 0.2

1.9 ± 0.5

0.6 ± 0.2

0.4 ± 0.1*

20 °C

0.8 ± 0.2*

0.3 ± 0.1

0.2 ± 0.0

0.3 ± 0.1

25 °C

0.6 ± 0.1

1.5 ± 0.5

1.4 ± 0.5

2.4 ± 0.4

Intestine

CCK

NC

6.2 ± 1.3

4.0 ± 1.8

3.5 ± 1.4

3.0 ± 1.2

20 °C

0.9 ± 0.2

1.1 ± 0.4

2.2 ± 0.9

1.1 ± 0.4

25 °C

46.7 ± 15.3

39.7 ± 11.1

42.2 ± 9.5

39.5 ± 13.5

  1. NPY neuropeptide Y, CCK cholecystokinin, LepA leptin-a, LepB leptin-b, NC natural condition, +0 h immediate time point after food intake, +3 h 3 hours after feeding, +6 h 6 hours after feeding, − 3 h 3 hours before feeding. All values represent the mean ± SEM: *P < 0.05; **P < 0.01