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Table 1 Steepness of absorbance kinetic (k) and luminescence value measured for absorbance at 0.055 (RLU) estimated for various concentration of palauolide (1) and Vibrio harveyi strains

From: Potential of fascaplysin and palauolide from Fascaplysinopsis cf reticulata to reduce the risk of bacterial infection in fish farming

Strain

Concentration of palauolide

Replicate

k

RLU

BB120

Control

1

0.0093

173985

  

2

0.0088

173717

  

3

0.0077

169546

  

All

0.0086 ( ± 0.0008)

172416 ( ± 2489)

 

C4

1

0.0100

173727

  

2

0.0104

174212

  

3

0.0093

172441

  

All

0.0099 ( ± 0.0006)

173460 ( ± 915)

 

C3

1

0.0105

178786

  

2

0.0116

179795

  

3

0.0107

179754

  

All

0.0110 ( ± 0.0006)

179445 ( ± 571)

 

C2

1

0.0119

174835

  

2

0.0116

178950

  

3

0.0117

178843

  

All

0.0117 ( ± 0.0001)

177542 ( ± 2345)

 

C1

1

0.0122

107211

  

2

0.0131

81339

  

3

0.0129

130080

  

All

0.0127 ( ± 0.0005)*

106210 ( ± 24385)

JAF 375

Control

1

0.0144

131953

  

2

0.0140

134519

  

3

0.0135

131708

  

All

0.0139 ( ± 0.0005)

132727 ( ± 1557)

 

C4

1

0.0160

129253

  

2

0.0168

131105

  

3

0.0183

130501

  

All

0.0170 ( ± 0.0012)

130286 ( ± 944)

 

C3

1

0.0151

146894

  

2

0.0154

146197

  

3

0.0171

145802

  

All

0.0159 ( ± 0.0011)

146298 ( ± 553)

 

C2

1

0.0172

140852

  

2

0.0163

141282

  

3

0.0160

140905

  

All

0.0165 ( ± 0.0006)

141014 ( ± 235)

 

C1

1

0.0170

159632

  

2

0.0179

158643

  

3

0.0165

158767

  

All

0.0171 ( ± 0.0007)

159014 ( ± 539)

JMH 597

Control

1

0.0094

147880

  

2

0.0081

146686

  

3

0.0100

147955

  

All

0.0091 ( ± 0.0010)

147507 (± 712)

 

C4

1

0.0120

150354

  

2

0.0118

147321

  

3

0.0121

147103

  

All

0.0119 ( ± 0.0001)

148259 ( ± 1818)

 

C3

1

0.0132

154515

  

2

0.0138

152906

  

3

0.0140

153875

  

All

0.0137 ( ± 0.0004)

153765 ( ± 810)

 

C2

1

0.0129

152211

  

2

0.0123

151867

  

3

0.0122

152261

  

All

0.0125 ( ± 0.0003)

152113 ( ± 214)

 

C1

1

0.0159

150802

  

2

0.0183

161802

  

3

0.0145

157737

  

All

0.0162 ( ± 0.0019)

156780 ( ± 5562)

JMH 612

Control

1

0.0057

186864

  

2

0.0083

192076

  

3

0.0087

189235

  

All

0.0076 ( ± 0.0017)

189392 ( ± 2609)

 

C4

1

0.0074

186349

  

2

0.0076

189886

  

3

0.0087

189302

  

All

0.0079 ( ± 0.0007)

188512 ( ± 1896)

 

C3

1

0.0088

187819

  

2

0.0076

174498

  

3

0.0078

184672

  

All

0.0081 ( ± 0.0007)

182330 ( ± 6963)

 

C2

1

0.0089

190911

  

2

0.0086

188991

  

3

0.0089

188746

  

All

0.0088 ( ± 0.0002)

189549 ( ± 1186)

 

C1

1

0.0106

120398

  

2

0.0097

91967

  

3

0.0091

87053

  

All

0.0098 ( ± 0.0008)

99806 ( ± 18002)

  1. Control, C4, C3, C2, and C1, respectively, refer to palauolide (1) concentration at 0, 1, 5, 10, and 50 μg ml–1. Estimated values of k and RLU are provided for each replicate (n = 3) and for all replicates (mean ± standard deviation over the three replicated). Values significantly different from controls (α = 0.05) are marked with a star (*)