Table 3 Nucleotide sequences of the primers used to assay gene expression by real-time PCR
Target genes | Forward (5′–3′) | Reverse (5′–3′) | GenBank accession no. |
|---|---|---|---|
Pax-7 | TCCAGCTCGTCTATCTGCAG | TAAACAGGTCTGAGGCTGGG | XM_005459001.3 |
Igf–I | CACCCTCTCACTACTGCTGT | CACAGTACATCTCAAGGCGC | EU272149.1 |
Igf–II | TGTTTCCGTAGCTGTGACCT | ACTTCACGGTCACATGTTGC | EU272150.1 |
GH | CTGGTTGAGTCCTGGGAGTT | CAGGTGGTTAGTCGCATTGG | KT387598.1 |
Psna-α 5 | TGTCTGGCTGTGGAGAAGAG | ATCTTCCTCCCCAAACTGCA | XM_003441520.3 |
Capn-3 | TCCAGTTCTGGCGTTATGGT | CCACCAGTGAAGTCCTCCAT | XM_005477402.2 |
MyoD | GCCGGATATCTCTTTCCCCA | AGGAAGGGGAGGATGAGGAT | FJ907953.1 |
MyoG | TCCTAGGGAAGCTGGGATCT | CGGTACCTGTCACTCCAACT | NM_001279526.1 |
β-Actin | TAATAACAGAACGCAGCGCC | AGTGCGGCGATTTCATCTTC | EU887951.1 |