Regulation of adductor muscle growth by the IGF-1/AKT pathway in the triploid Pacific oyster, Crassostrea gigas

Table 1 Primer sequences for RT-PCR, amplicon size, PCR efficiencies, and GenBank accession numbers of the genes evaluated in this study. Primers were designed based on Crassostrea gigas sequences. F forward primer, R reverse primer, AT annealing temperature

Gene	Accession number	Sequence (5′–3′)	Amplicon size (bp)	PCR cycle	AT (°C)
EF1α	AB122066.1	(F) CCACTGGCCATCTCATTTAC	393	20	60
EF1α	AB122066.1	(R) TGTTGACACCAATGATGAGC	393	20	60
IGF-1	XM_011417420.2	(F) ATGGTTTGCCCTGTCTTGAG	336	25	55
IGF-1	XM_011417420.2	(R) AGATCCTTTCTTCTTGCGGC	336	25	55
IGF-IR	AJ535669.1	(F) TGAGGAGGGTGATGAGGATA	375	25	55
IGF-IR	AJ535669.1	(R) ATTGCACTGTAGGGATTGGA	375	25	55
IGF-ALS	XM_011417921.2	(F) ATGTCCAAAACAATGCGTCT	359	25	55
IGF-ALS	XM_011417921.2	(R) TCCAAAACGCGTAACTTTTC	359	25	55
Actin-2	EKC31894.1	(F) TTTCGCCGGAGATGATGCCC	434	20	60
Actin-2	EKC31894.1	(R) TGGGGACAGTGTGGGTGACA	434	20	60
Myosin	EKC37566.1	(F) TTTGGCTGGTGAGGCACAGG	544	20	60
Myosin	EKC37566.1	(R) TTTGCTGAGCTGGCGTTGGA	544	20	60
Troponin T	XM_020062462.1	(F) AGGAACGCGAGAAAGAACAA	375	20	60
Troponin T	XM_020062462.1	(R) TGATCCTCTGGGACAGGAAG	375	20	60
Troponin I	XM_011455869.2	(F) CCACCCTGGAGGAAGAAGTC	187	20	60
Troponin I	XM_011455869.2	(R) AAATTGCCACGGAAATCTGA	187	20	60
Tropomyosin	NM_001308906.1	(F) GCCATGAAAATGGAGAAGGA	381	20	60
Tropomyosin	NM_001308906.1	(R) GGCGTTATTGAGGTTTTCCA	381	20	60

ISSN: 2234-1757