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Fig. 3 | Fisheries and Aquatic Sciences

Fig. 3

From: Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells

Fig. 3

Effect of HSS on the NF-κB translocation in TNF-α-treated HUVECs. a Cells were pretreated with or without HSS for 1 h followed by TNF-α treatment for 30 min. The subunits of NF-κB/p65 were detected by using anti-NF-κB polyclonal antibody and Alexa Fluor® 488-conjugated secondary antibody. The nuclei were detected by DAPI staining and confocal microscopy was used to capture the images. b Cells were incubated with an indicated concentration of HSS for 1 h and exposed to TNF-α (10 ng/mL) for 30 min. Cytosolic and nucleus fractions were analyzed by Western blotting. c The cells pretreated with the indicated concentrations of HSS for 1 h were stimulated with TNF-α (10 ng/mL) for 30 min. ROS was determined by DCFH-DA with fluorescent analysis. The values are shown as the means ± SDs from three independent experiments. #P < 0.05 represents significant differences versus the control group. *P < 0.05 represents significant differences versus the TNF-α-only group

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