Fig. 4

Evaluation of SJB-SHE potential to inhibit the NF-κB pathway-associated proteins in LPS stimulated RAW 264.7 macrophages. Cytosolic NF-κB p50 and p65 phosphorylation levels (a); the intensity of protein expressions were quantified using ImageJ (b). Nuclear translocation levels of NF-κB p50 and p65 (c) and the intensity of NF-κB protein expressions in the nucleus (d). β-actin (cytosolic) and C-23 (nucleus) were used as internal controls. Results are expressed as the mean ± SD of three separate experiments. *p < 0.05 and **p < 0.01