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Fig. 3 | Fisheries and Aquatic Sciences

Fig. 3

From: Ethanol extract separated from Sargassum horneri (Turner) abate LPS-induced inflammation in RAW 264.7 macrophages

Fig. 3

Inhibitory effects of SJB-SHE on LPS-induced inflammation-associated protein in RAW 264.7 macrophages. Western blots used to determine iNOS and COX2 levels (a), the related expression of the bands were analyzed using ImageJ software (b). β-actin was used as internal control. Results are expressed as the mean ± SD of three separate experiments. The gene expression analysis of iNOS (c) and COX2 (d). The 2−ΔΔCt method was used to calculate the relative mRNA levels. Internal reference used in the experiment was GAPDH. Experiments were triplicated. mRNA significance relative to the non-treated control was calculated using the Mann-Whitney U test. *p < 0.05 and **p < 0.01

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