Table 2 PCR primers used for the detection of bacterial and viral pathogens

L. garvieae

pLG1

5′-CAT AAC AAT GAG AAT CGC-3′

Baeck et al. 2006

pLG2

5′-GCA CCC TCG CGG GTT G-3′

Y. ruckeri

YER8

5′-GCG AGG AGG AAG GGT TAA GTG-3′

Gibello et al. 1999

YER10

5′-GAA GGC ACC AAG GCA TCT CTG-3′

V. anguillarum

Van-ami8 F

5′-ACA TCA TCC ATT TGT TAC-3′

Gonzalez et al. 2003

Van-ami417 R

5′-CCT TAT CAC TAT CCA AAT TG-3′

V. alginolyticus

gyrB Fw

5′-GAG AAC CCG ACA GAA GCG AAG-3′

Zhou et al. 2007

gyrB Re

5′-CCT AGT GCG GTG ATC AGT GTT G-3′

V. harveyi

VH-4F

5′-GTG ATG AAG AAG CTT ATC GCG ATT-3′

Kim et al. 2014

VH-7R

5′-CGC CTT CTT CAG TTA ACG CAG G-3′

V. vulnificus

vvhA Fw

5′-TTC CAA CTT CAA ACC GAA CTA TGA-3′

Panicker et al. 2004

vvhA Re

5′-ATT CCA GTC GAT GCG AAT ACG TTG-3′

V. penaeicida

Fw

5′-AA TAT TGC ACA ATG GGC GC-3′

Saulnier et al. 2000

Re

5′-G CGC TTT ACG CCC AGT AAT TCC G-3′

P. damselae

PD-2F

5′-CAA GAC ATC ATC GAT GTG ATG CGT-3′

Kim et al. 2014

PD-2R

5′-GAA ACT TTA CCA TCT ACC ACT TTG-3′

European catfish virus (ECV)

Fw

5′-ATGCGCTCCTCCGGGTCAAAG-3′

OIE 2011

Re

5′-CATCATGAACGGCTCGATGG-3′

Infectious hematopoietic necrosis virus (IHNV)

IHNV-GF

5′-AGA GAT CCC TAC ACC AGA GAC-3′

Emmenegger et al. 2000

IHNV-GR

5′-GGT GGT GTT GTT TCC GTG CAA-3′

Viral nervous necrosis (VNN)

VNN F

5′-CGT GTC AGT CAT GTG TCG CT-3′

Nishizawa et al. 1996

VNN R

5′-CGA GTC AAC ACG GGT GAA GA-3′

Infectious spleen and kidney necrosis virus–like (ISKNV-like)

P1 F

5′-ATG TCT GCA ATC TCA GGT-3′

Xu et al. 2008

P2 R

5′-TTA CAG GAT AGG GAA GCC TG-3′

NP1 (NP nested)

5′-GCG TTT GAT GCG ATG GAG AC-3′

NP2 (NP nested)

5′-ACG GCA GAG ACA CGG TAG GC-3′