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Table 2 PCR primers used for the detection of bacterial and viral pathogens

From: Introduction of bacterial and viral pathogens from imported ornamental finfish in South Korea

Pathogen Primer name Sequence Reference
L. garvieae pLG1 5′-CAT AAC AAT GAG AAT CGC-3′ Baeck et al. 2006
pLG2 5′-GCA CCC TCG CGG GTT G-3′
Y. ruckeri YER8 5′-GCG AGG AGG AAG GGT TAA GTG-3′ Gibello et al. 1999
YER10 5′-GAA GGC ACC AAG GCA TCT CTG-3′
V. anguillarum Van-ami8 F 5′-ACA TCA TCC ATT TGT TAC-3′ Gonzalez et al. 2003
Van-ami417 R 5′-CCT TAT CAC TAT CCA AAT TG-3′
V. alginolyticus gyrB Fw 5′-GAG AAC CCG ACA GAA GCG AAG-3′ Zhou et al. 2007
gyrB Re 5′-CCT AGT GCG GTG ATC AGT GTT G-3′
V. harveyi VH-4F 5′-GTG ATG AAG AAG CTT ATC GCG ATT-3′ Kim et al. 2014
VH-7R 5′-CGC CTT CTT CAG TTA ACG CAG G-3′
V. vulnificus vvhA Fw 5′-TTC CAA CTT CAA ACC GAA CTA TGA-3′ Panicker et al. 2004
vvhA Re 5′-ATT CCA GTC GAT GCG AAT ACG TTG-3′
V. penaeicida Fw 5′-AA TAT TGC ACA ATG GGC GC-3′ Saulnier et al. 2000
Re 5′-G CGC TTT ACG CCC AGT AAT TCC G-3′
P. damselae PD-2F 5′-CAA GAC ATC ATC GAT GTG ATG CGT-3′ Kim et al. 2014
PD-2R 5′-GAA ACT TTA CCA TCT ACC ACT TTG-3′
European catfish virus (ECV) Fw 5′-ATGCGCTCCTCCGGGTCAAAG-3′ OIE 2011
Re 5′-CATCATGAACGGCTCGATGG-3′
Infectious hematopoietic necrosis virus (IHNV) IHNV-GF 5′-AGA GAT CCC TAC ACC AGA GAC-3′ Emmenegger et al. 2000
IHNV-GR 5′-GGT GGT GTT GTT TCC GTG CAA-3′
Viral nervous necrosis (VNN) VNN F 5′-CGT GTC AGT CAT GTG TCG CT-3′ Nishizawa et al. 1996
VNN R 5′-CGA GTC AAC ACG GGT GAA GA-3′
Infectious spleen and kidney necrosis virus–like (ISKNV-like) P1 F 5′-ATG TCT GCA ATC TCA GGT-3′ Xu et al. 2008
P2 R 5′-TTA CAG GAT AGG GAA GCC TG-3′
NP1 (NP nested) 5′-GCG TTT GAT GCG ATG GAG AC-3′
NP2 (NP nested) 5′-ACG GCA GAG ACA CGG TAG GC-3′