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Table 4 Classification of maturity stages for males B. altianalis

From: Optimizing selection of sexually mature Barbus altianalis for induced spawning: determination of size at sexual maturity of populations from Lake Edward and Upper Victoria Nile in Uganda

Stages

Description

Stage I: Immature

 

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Strand-like Y-shaped gonad and transparent. This stage is dominated by spermatogonia only

Stage II: Developing

Easily distinguishable as males and off-white colored gonads. The majority of the cells are spermatogonia, and spermatocytes but occasionally few groups of spermatids and very few spermatozoa are observed

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Stage III: Spawning capable

Large gonads, off-white coloration; dominated by lobules filled with spermatozoa

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Stage IV: Regressed

 

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Reduced in size with bloody perches. Other portions still look off-white. Microscopically they are large empty spaces with residual spermatozoa. However other portions may still have some viable spermatozoa.

Stage V: Regenerating

 

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Further reduced in size with pale reddish coloration. This stage largely contains parked spermatogonia with reduced spaces containing very few residual spermatozoa.