Table 1 Dietary formulation and proximate composition of the six experimental diets for L. vannamei (% dry matter). All three experimental (trial 1, 2, and 3) diets were produced using the same diet formulation
Ingredients | Experimental diets | |||||
|---|---|---|---|---|---|---|
P25 | P30 | P35 | P40 | P45 | P50 | |
Brown fish meal1 | 18.0 | 18.0 | 18.0 | 18.0 | 18.0 | 18.0 |
Gelatin2 | 0.50 | 1.50 | 2.50 | 3.50 | 4.50 | 5.50 |
Casein3 | 2.00 | 6.00 | 10.0 | 14.0 | 18.0 | 22.0 |
Soybean meal | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 |
Squid liver meal | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
Wheat flour | 34.5 | 34.5 | 34.5 | 34.5 | 34.5 | 34.5 |
Starch | 30.0 | 25.0 | 20.0 | 15.0 | 10.0 | 5.00 |
Fish oil | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
Vitamin/mineral premix4 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
Choline chloride | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
Lecithin | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
Chemical composition (% dry matter) | ||||||
Dry matter | 89.0 | 88.8 | 88.4 | 88.9 | 89.0 | 88.6 |
Crude protein | 25.9 | 30.4 | 35.8 | 40.2 | 45.0 | 50.1 |
Crude lipid | 8.12 | 8.09 | 8.18 | 8.17 | 8.27 | 8.23 |
Crude ash | 5.19 | 5.13 | 5.15 | 5.28 | 5.14 | 5.33 |