Dietary protein requirement of Pacific white shrimp Litopenaeus vannamei in three different growth stages

Table 1 Dietary formulation and proximate composition of the six experimental diets for L. vannamei (% dry matter). All three experimental (trial 1, 2, and 3) diets were produced using the same diet formulation

Ingredients	Experimental diets
Ingredients	P25	P30	P35	P40	P45	P50
Brown fish meal¹	18.0	18.0	18.0	18.0	18.0	18.0
Gelatin²	0.50	1.50	2.50	3.50	4.50	5.50
Casein³	2.00	6.00	10.0	14.0	18.0	22.0
Soybean meal	4.00	4.00	4.00	4.00	4.00	4.00
Squid liver meal	5.00	5.00	5.00	5.00	5.00	5.00
Wheat flour	34.5	34.5	34.5	34.5	34.5	34.5
Starch	30.0	25.0	20.0	15.0	10.0	5.00
Fish oil	2.00	2.00	2.00	2.00	2.00	2.00
Vitamin/mineral premix⁴	2.00	2.00	2.00	2.00	2.00	2.00
Choline chloride	1.00	1.00	1.00	1.00	1.00	1.00
Lecithin	1.00	1.00	1.00	1.00	1.00	1.00
Chemical composition (% dry matter)
Dry matter	89.0	88.8	88.4	88.9	89.0	88.6
Crude protein	25.9	30.4	35.8	40.2	45.0	50.1
Crude lipid	8.12	8.09	8.18	8.17	8.27	8.23
Crude ash	5.19	5.13	5.15	5.28	5.14	5.33

¹Suhyup Feed Co. Ltd., South Korea (crude protein: 73.1%, crude lipid: 6.60%)
²Sigma Chemicals, Gelatin
³Casein was purchased from USB Co. Ltd., Cleveland, OH, USA
⁴Vitamin/mineral premix (g kg⁻¹of mixture): retinol, 3.0; cholecalciferol, 1.0; ascorbic acid, 20.0; tocopherol, 20.0; menadione, 2.0; thiamine, 4.0; riboflavin, 6.0; pyridoxine, 5.0; cobalamin, 6.0; inositol, 54.0; panththenic acid, 12.0; biotin, 0.2; niacin amide, 40.0; folic acid, 2.0; Ferriccitrate, 10.0; Cu, 1.0; Zn, 30; Mn, 2.0; Co, 10.; I, 1.0; K, 6.0; Se, 0.01

ISSN: 2234-1757