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Table 1 Dietary formulation and proximate composition of the six experimental diets for L. vannamei (% dry matter). All three experimental (trial 1, 2, and 3) diets were produced using the same diet formulation
| Ingredients | Experimental diets | |||||
|---|---|---|---|---|---|---|
| P25 | P30 | P35 | P40 | P45 | P50 | |
| Brown fish meal1 | 18.0 | 18.0 | 18.0 | 18.0 | 18.0 | 18.0 |
| Gelatin2 | 0.50 | 1.50 | 2.50 | 3.50 | 4.50 | 5.50 |
| Casein3 | 2.00 | 6.00 | 10.0 | 14.0 | 18.0 | 22.0 |
| Soybean meal | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 | 4.00 |
| Squid liver meal | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Wheat flour | 34.5 | 34.5 | 34.5 | 34.5 | 34.5 | 34.5 |
| Starch | 30.0 | 25.0 | 20.0 | 15.0 | 10.0 | 5.00 |
| Fish oil | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Vitamin/mineral premix4 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
| Choline chloride | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Lecithin | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Chemical composition (% dry matter) | ||||||
| Dry matter | 89.0 | 88.8 | 88.4 | 88.9 | 89.0 | 88.6 |
| Crude protein | 25.9 | 30.4 | 35.8 | 40.2 | 45.0 | 50.1 |
| Crude lipid | 8.12 | 8.09 | 8.18 | 8.17 | 8.27 | 8.23 |
| Crude ash | 5.19 | 5.13 | 5.15 | 5.28 | 5.14 | 5.33 |
