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Table 1 Dietary formulation and proximate composition of the six experimental diets for L. vannamei (% dry matter). All three experimental (trial 1, 2, and 3) diets were produced using the same diet formulation

From: Dietary protein requirement of Pacific white shrimp Litopenaeus vannamei in three different growth stages

Ingredients Experimental diets
P25 P30 P35 P40 P45 P50
Brown fish meal1 18.0 18.0 18.0 18.0 18.0 18.0
Gelatin2 0.50 1.50 2.50 3.50 4.50 5.50
Casein3 2.00 6.00 10.0 14.0 18.0 22.0
Soybean meal 4.00 4.00 4.00 4.00 4.00 4.00
Squid liver meal 5.00 5.00 5.00 5.00 5.00 5.00
Wheat flour 34.5 34.5 34.5 34.5 34.5 34.5
Starch 30.0 25.0 20.0 15.0 10.0 5.00
Fish oil 2.00 2.00 2.00 2.00 2.00 2.00
Vitamin/mineral premix4 2.00 2.00 2.00 2.00 2.00 2.00
Choline chloride 1.00 1.00 1.00 1.00 1.00 1.00
Lecithin 1.00 1.00 1.00 1.00 1.00 1.00
Chemical composition (% dry matter)
 Dry matter 89.0 88.8 88.4 88.9 89.0 88.6
 Crude protein 25.9 30.4 35.8 40.2 45.0 50.1
 Crude lipid 8.12 8.09 8.18 8.17 8.27 8.23
 Crude ash 5.19 5.13 5.15 5.28 5.14 5.33
  1. 1Suhyup Feed Co. Ltd., South Korea (crude protein: 73.1%, crude lipid: 6.60%)
  2. 2Sigma Chemicals, Gelatin
  3. 3Casein was purchased from USB Co. Ltd., Cleveland, OH, USA
  4. 4Vitamin/mineral premix (g kg−1of mixture): retinol, 3.0; cholecalciferol, 1.0; ascorbic acid, 20.0; tocopherol, 20.0; menadione, 2.0; thiamine, 4.0; riboflavin, 6.0; pyridoxine, 5.0; cobalamin, 6.0; inositol, 54.0; panththenic acid, 12.0; biotin, 0.2; niacin amide, 40.0; folic acid, 2.0; Ferriccitrate, 10.0; Cu, 1.0; Zn, 30; Mn, 2.0; Co, 10.; I, 1.0; K, 6.0; Se, 0.01