Fisheries and Aquatic Sciences

Table 2 Effects of different clove oil concentrations and temperatures on anesthesia time and recovery time of juvenile and adult red spotted grouper, Epinephelus akaara*

From: Anesthetic efficacy and physiological responses of clove oil on juvenile and adult red spotted grouper, Epinephelus akarra

Temperature (°C)	Dose (ppm)	Time (sec)
		Juvenile		Adult
		Exposure	Recovery	Exposure	Recovery
20	25	151 ± 17.2^c	291 ± 23.5^b	255 ± 15.4^c	280 ± 21.7^b
	50	103 ± 10.4^b	273 ± 17.3^b	163 ± 11.7^b	271 ± 19.3^b
	75	75 ± 10.3^a	238 ± 16.5^a	107 ± 9.5^a	243 ± 11.5^a
24	25	138 ± 9.3^c	288 ± 21.8^b	221 ± 15.6^c	257 ± 20.3^b
	50	91 ± 10.1^b	256 ± 18.1^a	123 ± 9.3^b	232 ± 17.2^a
	75	67 ± 9.4^a	241 ± 15.7^a	88 ± 6.2^a	214 ± 15.1^a
28	25	126 ± 10.5^c	267 ± 24.9^b	153 ± 10.5^c	248 ± 21.5^b
	50	83 ± 11.2^b	273 ± 20.1^b	94 ± 11.2^b	219 ± 16.8^b
	75	59 ± 7.9^aA	229 ± 12.1^a	71 ± 9.9^a	83 ± 9.1^a

*Each value is mean ± standard deviation of triplicate independent experiments (n = 10). Different small letters indicate significant differences for the different clove oil dose at the same water temperature (P < 0.05), the same superscript letter are not significantly different (P > 0.05)

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