Fig. 4

Effect of D. puetteri ethanolic extract on tyrosinase and ERK pathway associated protein expression in α-MSH-stimulated B16F10 cells. Cells were incubated with α-MSH (100 nM) together with DPE (200 μg/mL) for 48 h in the absence or presence of PD98059 (10 μM). a Tyrosinase, b ERK, and p-ERK protein expressions were visualized by Western blotting. Data points and bars represent the arithmetic means ± SEM (n = 3) (*p < 0.05; **p < 0.01)