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Fig. 3 | Fisheries and Aquatic Sciences

Fig. 3

From: Anti-inflammatory effect of ozonated krill (Euphausia superba) oil in lipopolysaccharide-stimulated RAW 264.7 macrophages

Fig. 3

Effect of ozonated krill oil on LPS-stimulated expression of TNF-α, IL-1β, and IL-6 in RAW 264.7 macrophages. Cells were stimulated with LPS (1 μg/mL) in the presence of ozonated krill oil (10, 50, and 100 μg/mL) for 24 h at 37 °C. The expression of levels of pro-inflammatory cytokines mRNA were measured by RT-PCR analysis. TNF-α, IL-1β, and IL-6 versus GAPDH were measured via densitometry. The density ratio of the mRNA expressed in the LPS-stimulated group was considered to represent onefold. The data is expressed as mean folds of the protein and mRNA expression versus LPS-stimulated group. Values are expressed as means ± S.D. of triplicate experiments. *p < 0.05 and **p < 0.01 indicate significant differences from the LPS-stimulated group

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