Fig. 2

Effect of ozonated krill oil on LPS-stimulated expression of iNOS and COX-2 in RAW 264.7 macrophages. Cells were stimulated with LPS (1 μg/mL) in the presence of ozonated krill oil (10, 50, and 100 μg/mL) for 24 h at 37 °C. The expression of levels of iNOS and COX-2 a protein and b mRNA were measured using western blot and by RT-PCR analysis, respectively. iNOS and COX-2 versus GAPDH were measured by a densitometer. The density ratio of the protein and mRNA expressed in the LPS-stimulated group were considered to represent onefold. The data is expressed as mean folds of the protein and mRNA expression versus LPS-stimulated group. Values are expressed as means ± S.D. of triplicate experiments. ^*p < 0.05 and ^**p < 0.01 indicate significant differences from the LPS-stimulated group