Fig. 4

Inhibitory effect of the JFH on the protein level of p38, ERK, and JNK in RAW264.7 macrophages. Cells were stimulated with LPS (0.25 μg/ml) in the presence of JFH (10, 50, and 100 μg/ml) for 30 min. The expression level of p38, JNK, and ERK were measured by Western blotting analysis. The density ratio of the protein expressed in the LPS-induced group was considered to represent 1-fold. The data is expressed as mean folds of the protein expression versus LPS-stimulated group. Values are expressed as means ± S.D. of triplicate experiments. *p < 0.05 indicates significant differences from the LPS-stimulated group