Fig. 3

Inhibitory effect of the JFH on LPS-induced IL-1β, IL-6, and TNF-α production in RAW264.7 macrophages. Cells were stimulated with LPS (0.25 μg/ml) in the presence of JFH (10, 50, and 100 μg/ml) for 24 h. The levels of IL-1β, IL-6, and TNF-α mRNA were determined by RT-PCR analysis. The values are expressed as the mean ± S.D. of triplicate experiments. *p < 0.05 indicates significant differences from the LPS-stimulated group