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Fig. 2 | Fisheries and Aquatic Sciences

Fig. 2

From: The pepsinolytic hydrolysate from Johnius belengerii frame inhibited LPS-stimulated production of pro-inflammatory mediators via the inactivating of JNK and NF-κB pathways in RAW 264.7 macrophages

Fig. 2

Inhibitory effect of the JFH in LPS-induced iNOS and COX-2 protein and mRNA expression levels in RAW264.7 macrophages. Cells were stimulated with LPS (0.25 μg/ml) in the presence of JFH (10, 50, and 100 μg/ml) for 24 h. The expression levels of iNOS and COX-2 protein (a) and mRNA (b) were measured by Western blotting and RT-PCR analysis, respectively. iNOS and COX-2 versus GAPDH were measured by densitometer. The density ratio of the protein and mRNA expressed in the LPS-stimulated group were considered to represent 1-fold. The data is expressed as mean folds of the protein and mRNA expression versus LPS-stimulated group. Values are expressed as means ± S.D. of triplicate experiments. *p < 0.05 indicates significant differences from the LPS-stimulated group

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