Fig. 2
The steps for purification of β-secretase inhibitory peptide from sea hare muscle hydrolysate. (I) Sephadex G-25 Gel filtration chromatogram of hydrolysates. (II, III, IV) Reverse phase-HPLC chromatograms of the potent β-secretase inhibitory fractions from the previous steps. The lower panels (a) of each pair show the chromatography results of the separated fractions while the top panels (b) of each pair represent the β-secretase inhibitory activity of the separated fractions in terms of their IC50 values expressed in μg/ml. Statistical significance was determined by ANOVA (Duncan’s test, mean ± SD; n = 3). ANOVA test results are shown as letters above columns. Means not sharing the same letter are significantly different