Fig. 1

Developmental toxicity of SCDE in zebrafish embryos. a Survival rates throughout 24–120 hpf. b Heart beating rates at 48 hpf. The values are expressed as the mean ± SE. Significant differences from the untreated group were identified at *P < 0.05 and **P < 0.01. c Representative images of developmental malformations in zebrafish embryos exposed to indicated concentration of SCDE at 24 and 48 hpf. d Effect of SCDE on cell death in zebrafish embryos. The cell death levels were measured after acridine orange staining by image analysis and fluorescence microscope. Representative fluorescence micrographs of cell death level, and data obtained from individual zebrafish fluorescence intensity analyses using an image J program are shown as bar graph. The values are expressed as the mean ± SE