Fig. 3

RT-qPCR analysis of mud loach ACTA1 (a), ACTC1 (b), and ACTA2 (c) transcripts in the brain (Br), eye (Ey), fin (Fi), gill (Gi), heart (He), intestine (In), kidney (Ki), liver (Li), skeletal muscle (Mu), spleen (Sp), skin (Sk), ovary (Ov), and testis (Te). Expression levels of each isoform were normalized against 18S rRNA control. Histograms excluding the muscle (for ACTA1), heart (for ACTC1), and intestine (for ACTA2) are also provided in a′, b′, and c′, respectively, for better resolution of expressed levels in other tissues. Within each isoform, means (± s.d.) with different letters indicate statistical difference based on one-way ANOVA followed by Duncan’s multiple ranged tests (P < 0.05). In b′, tissues showing undetectable mRNA expression levels of ACTC1 are indicated by nd