Effects of different algae in diet on growth and interleukin (IL)-10 production of juvenile sea cucumber Apostichopus japonicus

Table 1 Ingredients and composition of experimental diets for Apostichopus japonicus (% dry matter basis)

Ingredients	ST (control)	UL	UP	LJ	SS	NO
Ulva lactuca powder	0	15	0	0	0	0
Undaria pinnatifida powder	0	0	15	0	0	0
Laminaria japonica powder	0	0	0	15	0	0
Schizochytrium sp. powder	0	0	0	0	15	0
Nannochloropsis oculata powder	0	0	0	0	0	15
Wheat flour	15	0	0	0	0	0
Seaweed powder	15	15	15	15	15	15
Soybean meal	8	8	8	8	8	8
Shellfish powder	8	8	8	8	8	8
Shell powder	2	2	2	2	2	2
Calcium phosphate	2	2	2	2	2	2
Yeast protein	5	5	5	5	5	5
Soyabean lecithin	4	4	4	4	4	4
Mineral premix^a	0.5	0.5	0.5	0.5	0.5	0.5
Vitamin premix^b	0.5	0.5	0.5	0.5	0.5	0.5
Sea mud	40	40	40	40	40	40
Proximate composition (%)
Crude protein	17.74	17.03	17.48	16.87	17.59	19.64
Crude lipid	3.39	3.72	3.11	3.32	7.44	5.64
Ash	41.20	44.80	45.55	45.85	41.5	42.10

^aMineral premix (g kg⁻¹ premix): MgSO₄·7H₂O, 80.0; NaH₂PO₄·2H₂O, 200.0; KH₂PO₄, 130.0; Ferric citrate, 40.0; ZnSO₄·7H₂O, 10; Ca-lactate, 25.5; CuCl, 0.2; AlCl₃·6H₂O, 0.15; KIO₃, 0.15; Na₂Se₂O₃, 0.01; MnSO₄·H₂O, 2.0; CoCl₂·6H₂O, 1.0
^bVitamin premix (g kg⁻¹ premix): ascorbic acid, 92.7; α-tocopheryl acetate, 14.5; thiamine hydrochloride, 7; riboflavin, 7.0; pyridoxine hydrochloride, 1.4; niacin, 27.8; Ca-D-pantothenate, 9.7; myo-inositol, 139.1; D-biotin, 0.5; folic acid, 0.5; p-amino benzoic acid, 13.9; menadione, 4.2; retinyl acetate, 0.65; cholecalciferol, 0.8; cyanocobalamin, 0.004

ISSN: 2234-1757