Table 1 List of oligonucleotide primers used in this study
Primer name | Sequence (5′-3′) | Thermal cycling conditions | Application |
|---|---|---|---|
mmAQP1a FW | CAGAAATCTCCACATTACCAGC | 35 cycles at 94 °C for 20 s, 58 °C for 20 s, 72 °C for 1.5 min, followed by a final elongation at 72 °C for 3 min | Isolation of mud loach aquaporin cDNAs |
mmAQP1a RV | TCCTGAGGTACATACTGATTC | ||
mmAQP3a FW | ACACACGTTCAAGGGAAAGC | ||
mmAQP3a RV | CTGGCTGGATTCACAGCATA | ||
mm18S rRNA RV | GGTTTCCCGTGTTGAGTCAA | Reverse transcription at 37 °C for 60 min | Preparation of normalization control |
q-mmAQP1a FW | ATGAGAGTGCTGGTCTCTGG | 45 cycles at 94 °C for 20 s, 60 °C for 20 s and 72 °C for 20 s | Real-time PCR assay of mud loach aquaporin mRNAs Real-time PCR assay of 18S rRNA control |
q-mmAQP1a RV | AAAGACAGCTTCACAATTGC | ||
q-mmAQP3a FW | TATGGAGGAGAATGTGAAGC | ||
q-mmAQP3a RV | AATCTGGAGATGTGCAGCGT | ||
q-mm18S rRNA FW | AAGCTCGTAGTTGGATCTCG | ||
q-mm18S rRNA RV | CCTAGCTGCGGTATTCAGGC |