Fig. 1

Multiple amino acid sequence alignments of mud loach Misgurnus mizolepis AQP isoforms 1a (a) and 3a (b) along with those from other selected vertebrate species. Asterisks and hyphens indicate identical residues and gaps introduced for optimum alignments, respectively. The arrowhead above mmAQP1a showed the conserved residues Phe⁵⁶, His¹⁸⁰, and Arg¹⁹⁵ (mud loach AQP1a numbering). Two NPA motifs are shown in bold letters. The putative transmembrane locations of membrane-spanning domains are indicated above the alignment. The gray histogram below the ruler depicts sequence conservation between amino acid residues in given AQP isoforms