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Table 1 Formulation and chemical analysis of the five experimental diets for olive flounder (P. olivaceus) (%, dry matter)

From: Taurine supplementation in diet for olive flounder at low water temperature

Ingredients Experimental diets
Control T1 T2 T3 T4
 Brown fish meal 50.0 50.0 50.0 50.0 50.0
 Soybean meal 5.00 5.00 5.00 5.00 5.00
 Corn gluten meal 5.00 5.00 5.00 5.00 5.00
 Wheat flour 27.0 27.0 27.0 27.0 27.0
 Fish oil 8.00 8.00 8.00 8.00 8.00
 Starch 1.00 1.00 1.00 1.00 1.00
 Mineral mixa 1.00 1.00 1.00 1.00 1.00
 Vitamin mixb 1.00 1.00 1.00 1.00 1.00
 Choline chloride 0.50 0.50 0.50 0.50 0.50
 Glycine 1.50 1.25 1.00 0.50 0.00
 Taurine 0.00 0.25 0.50 1.00 1.50
Chemical analysis (%, dry matter)
 Moisture 6.9 7.2 7.1 7.0 7.1
 Crude protein 48.9 48.8 49.2 49.0 49.0
 Crude lipid 13.2 13.0 13.1 13.0 13.2
 Crude ash 8.0 8.1 8.1 8.2 8.2
 Taurine 0.12 0.33 0.42 0.92 1.34
  1. aMineral premix (g/kg mixture): MgSO4.7H2O, 80.0; NaH2PO4.2H2O, 370.0; KCl, 130.0; Ferric citrate, 40.0; ZnSO4.7H2O, 20.0; Ca-lactate, 356.5; CuCl2, 0.2; AlCl3. 6H2O, 0.15; Na2Se2O3, 0.01; MnSO4.H2O, 2.0; CoCl2.6H2O, 1.0
  2. bVitamin premix (g/kg mixture): L-ascorbic acid, 121.2; DL-a tocopheryl acetate, 18.8; thiamin hydrochloride, 2.7; riboflavin, 9.1; pyridoxine hydrochloride, 1.8; niacin, 36.4; Ca-D-pantothenate, 12.7; myo-inositol, 181.8;D-biotin, 0.27; folic acid, 0.68; p-aminobezoic acid, 18.2; menadione, 1.8; retinyl acetate, 0.73; cholecalficerol, 0.003; cyanocobalamin, 0.003