Table 1 Detection of K. septempunctata DNA in rearing water samples from fish farms by molecular methods
Sampling date | PCR | Real-time PCR | ||||
|---|---|---|---|---|---|---|
Ct value | rDNA copy number | |||||
Farm A | Farm B | Farm A | Farm B | Farm A | Farm B | |
2014. May. | – | – | 40.9, 41.1 | 40.1, 43.0 | 9.0, 7.7 | 16.3, 1.9 |
2014. Jun. | – | – | ND, ND | 42.2, 40.4 | ND, ND | 3.4, 13.1 |
2014. Jul. | – | – | ND, ND | 36.1, 38.3 | ND, ND | 3.2 × 102, 62.5 |
2014. Aug. | – | – | 42.2, ND | ND, ND | 3.5, ND | ND, ND |
2014. Sept. | – | – | 41.4, 39.3 | 40.7, ND | 6.2. 29.7 | 10.4, ND |
2014. Oct. | – | – | 36.3, 34.5 | 37.0, 37.9 | 2.8 × 102, 1.1 × 103 | 1.6 × 102, 87.5 |
2014. Nov. | – | – | 40.0, 39.5 | 39.3, 39.8 | 18.3, 26.2 | 29.7, 20.4 |