Fig. 2From: Circadian rhythm of melatonin secretion and growth-related gene expression in the tiger puffer Takifugu rubripes Tissue-specific expression of growth-related genes in the portion of the brain of tiger puffer. The brain was further divided into five parts. They were analyzed by RT-PCR (a) and qPCR (b–d). The expression of β-actin mRNA was used as reference. Te telencephalon, Op optic tectum, Di diencephalon, Ce cerebellum, Me medulla oblongata, N.C negative control, M 100 bp DNA ladder marker. Means represented by different letters are significant (P < 0.05). Values are mean ± SEMBack to article page