Fig. 6

Differential mRNA expression patterns of abalone MTF-1 in response to heat shock treatments. During heat elevation with an increase rate of 1 °C/h, abalones were sampled at 20 °C (just before the elevation), 25 °C, and 30 °C. After reaching 30 °C, abalones were exposed at the constant 30 °C and sampled after 12 h (30 °C+12 h) and 24 h (30 °C+24 h), respectively. RT-qPCR data were presented as fold changes of MTF-1 mRNAs in expressed groups relative to that of non-heat shocked group (i.e., 20 °C control) based on the normalization with RPL5 and PPL7 reference genes. Histograms with different letters indicate the significantly different means based on ANOVA followed by Duncan’s multiple ranged tests at P < 0.05