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Table 1 Incidence of naturally occurring RFP signals in the liver of transgenic marine medaka with the function of age

From: Transgene chgH-rfp expression at developmental stages and reproductive status in marine medaka (Oryzias dancena)

Age (weeks post hatching) No. of TG fish examined Average total length (mm) % RFP-positive fish (no. of RFP-positive fish/no. of TG fish)
Female Male Not sexed
Trial I
1 75 7.9 ± 0.2 0.0 (0/75)
2 74 10.2 ± 0.4 0.0 (0/74)
3 79 10.8 ± 0.4 0.0 (0/79)
4 81 12.9 ± 0.6 0.0 (0/81)
5 72 15.2 ± 1.0 0.0 (0/72)
6 79 17.5 ± 1.2 0.0 (0/39) 0.0 (0/40)
7 82 19.1 ± 1.5 7.7 (3/39) 0.0 (0/43)
8 85 21.8 ± 1.4 47.6 (20/42) 0.0 (0/43)
9 76 23.1 ± 2.1 75.0 (27/36) 0.0 (0/40)
10 87 25.2 ± 2.2 95.6 (43/45) 0.0 (0/42)
Trial II
1 85 7.5 ± 0.2 0.0 (0/85)
2 83 9.9 ± 0.3 0.0 (0/83)
3 88 10.2 ± 0.5 0.0 (0/88)
4 85 12.5 ± 0.5 0.0 (0/85)
5 87 15.0 ± 1.0 0.0 (0/87)
6 85 17.1 ± 1.2 0.0 (0/40) 0.0 (0/45)
7 79 19.3 ± 1.3 7.5 (3/40) 0.0 (0/39)
8 81 20.9 ± 1.5 55.0 (22/40) 0.0 (0/41)
9 86 22.8 ± 2.0 70.7 (29/41) 0.0 (0/45)
10 85 24.8 ± 2.1 100.0 (44/44) 0.0 (0/41)
  1. Number of transgenic fish was estimated based on the PCR typing of RFP-negative fish. Gonadic sex of fish at 6 WPH and 7 WPH was judged based on the microscopic examination of squashed gonad specimens, while sexing of the fish at 8 WPH to 10 WPH was done by direct examination of gonads with the unaided eye