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Table 3 Primers and probes used for one-step quantitative real-time RT-PCR

From: Butyrate and taurine exert a mitigating effect on the inflamed distal intestine of European sea bass fed with a high percentage of soybean meal

Gene

Nucleotide sequence (5′–3′)

IL-1β

F: TTGTGTTTGAGCGCGGAACA

R: TGTCGGTCACGCTGCATTG

Probe: 6-Fam-CTCCAACAGCGCAGTACAGCAAGC-BHQ1

IL-6

F: GCCTGCTCACTTACACAGCTCTTC

R: TCTTGAAACTGTGGCCCTCTGA

Probe: 6-Fam-AGAAGGAGTCCCCCAGCTCGATCCG-BHQ1

IL-8

F: CTGTCGCTGCATCCAAACAGA

R: GCAATGGGAGTTAGCAGGAATCAG

Probe: 6-Fam-AGCAAACCCATCGGCCGCCAC-BHQ1

IL-10

F: AGCGCTGCTAGACCAGACTGT

R: CGGCAGAACCGTGCTTAGAT

Probe: 6-Fam-AGACACTTTAAAGACCCCGTTCGCTTGC-BHQ1

TNFα

F: AAACCGGCCTCTACTTCGTCTA

R: TCCCGCACTTTCCTCTTCAC

Probe: 6-Fam-AGCCAGGCGTCGTTCAGAGTCTCC-BHQ1