Table 2 Sequences and melting temperatures (Tm) of the primers used for in vitro synthesis of standard mRNAs
Gene | GenBank acc. nr. | Nucleotide sequence (5′–3′) | Tm (°C) |
|---|---|---|---|
IL-1β | AJ269472 | F: gtaatacgactcactatagggTGCCATGGAGAGACTGAAGG | >70 |
R: ACTGGGTGTACGGTCCAAGT | 60.7 | ||
IL-6 | AM490062 | F: gtaatacgactcactatagggACTTCCAAAACATGCCCTGA | >70 |
R: CCGCTGGTCAGTCTAAGGAG | 59.5 | ||
IL-8 | AM490063 | F: gtaatacgactcactatagggTCAGTGAAGGGATGAGTCTGA | >70 |
R: CTCGGGGTCCAGGCAAAC | 60.3 | ||
IL-10 | AM268529 | F: gtaatacgactcactatagggCAGTGCTGTCGTTTTGTGGA | >70 |
R: TCACTCTTGAGCTGGTCGAAG | 59.7 | ||
TNFα | DQ070246 | F: gtaatacgactcactatagggCACTACACACTGAAGCGCAT | >70 |
R: CTGTAGCTGTCCTCCTGAGC | 59.5 |