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Fig. 1 | Fisheries and Aquatic Sciences

Fig. 1

From: Cloning, expression, and activity of type IV antifreeze protein from cultured subtropical olive flounder (Paralichthys olivaceus)

Fig. 1

Multiple sequence alignment of AFP IVs. Sequence alignment was carried out using ClustalW2 (Larkin et al. 2007). Presented are only mature protein sequences from Paralichthys olivaceus (Pol, GenBank Accession. No. Q8JI37); Myoxocephalus octodecemspinosus (Moc, ABA41379); Myoxocephalus octodecemspinosus (Moc, ABA41379); Notothenia coriiceps (Nco, ADU02181); Pleuragramma antarcticum (Pan, ADU02180); Danio rerio (Dre_A, NP_001038953 and Dre_B, XP_697091); and Carassius gibelio (Cgi_A, AHZ08737 and Cgi_B, AAR12991). Amino acid consensus is shown below the alignment via three kinds of consensus symbols. An asterisk (*) indicates a position that has a single conserved residue; a colon (:) indicates a position that exhibits high similarity (scoring >0.5); and a period (.) indicates a position that shows low similarity (scoring ≤0.5). Asterisks (*) above protein sequences note the predicted α-helical regions. Dotted boxes indicate the four helices (A, B, C, and D) of the antiparallel bundle model of AFP IV. CD is a linker between the C and D helices

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